ID 31845
file
title alternative
パーティクルガン法による被子植物花粉の形質転換に関する研究
creator
Nishihara, Masahiro
NDC
Botany
contents
CONTENTS
INTRODUCTION / p1
CHAPTER I GENE DELIVERY AND EXPRESSION IN POLLEN CELLS BY PARTICLE BOMBARDMENT / p4
 Section1 Transient expression of the β-glucuronidase gene in pollen by particle bombardment / p4
 Section2 Optimization of bombardment conditions for pollen cells / p11
 Section3 Comparison of promoter activities of pollen-expressed genes in pollen of mono- and dicot plants / p16
 Section4 Cytological observation of GUS-expressing pollen of lily / p23
CHAPTER II TRANSGENIC HAPLOID PLANTS PRODUCED BY BOMBARDMENT-MEDIATED TRANSFORMATION / p29
 Section1 Regeneration of pollen-derived transgenic haploid plants of Nicotiana rustica / p29
 Section2 Analyses of transgenic haploid plants of Nicotiana rustica / p35
CONCLUSIONS / p44
ACKNOWLEDGMENTS / p46
REFERENCES / p48
LIST OF PUBLICATIONS / p57
SelfDOI
language
eng
nii type
Thesis or Dissertation
HU type
Doctoral Theses
DCMI type
text
format
application/pdf
text version
ETD
rights
Copyright(c) by Author
relation references
・Nishihara, M., Ito, M., Tanaka, I., Kyo, M., Ono, K., Irifune, K., and Morikawa, H. (1993) Expression of the β-glucuronidase gene in pollen of lily (Lilium longiflorum), tobacco (Nicotiana tabacum), Nicotiana rustica, and peony (Paeonia lactiflora) by particle bombardment. Plant Physiol. 102: 357-361.
・Nishihara, M., Seki, M., Kyo, M., Irifune, K., and Morikawa, H. Transgenic haploid plants of Nicotiana rustica produced by bombardment-mediated transformation. Transgenic Res. (in press).
relation references URL
http://dx.doi.org/10.1104/pp.102.2.357
http://dx.doi.org/10.1007/BF01972531
grantid
甲第1300号
degreeGrantor
広島大学(Hiroshima University)
degreename Ja
博士(理学)
degreename En
Physical Science
degreelevel
doctoral
date of granted
1994-11-28
department
Graduate School of Science