ID 31856
file
title alternative
有用タンパク質の生産、構造解析と機能改変
creator
Yamashita, Mitsuo
NDC
Biology
contents
TABLE OF CONTENTS
PREFACE / p1
Chapter I Expression of a Gene for Human Protein:Genetic Design of a Gene for Human Metallothionein II and Its Expression as an Active Fusion Protein in Escherichia coli / p9
 1.Introduction / p10
 2.Materials and methods / p11
 3.Results / p15
 4.Discussion / p24
 5.Summary / p27
 6.References / p28
Chapter II Production and Purification of Monoamine Oxidase:Production, Purification and Characterization of a new type of Monoamine Oxidase containing Cu from Klebsiella aerogenes / p32
 1.Introduction / p33
 2.Materials and methods / p35
 3.Results / p39
 4.Discussion / p51
 5.Summary / p54
 6.References / p55
Chapter III Structure and Function of Pullulanase:Role of Lipid Modification on a Starch-debranching Enzyme,Klebsiella Pullulanase / p60
 1.Introduction / p61
 2.Materials and methods / p63
 3.Results / p65
 4.Discussion / p75
 5.Summary / p77
 6.References / p78
Chapter IV Functinal Development of Pullulanase:Random mutagenesis of Pullulanase from Klebsilella aerogenes for Studies of the Structure and Function of the Enzyme / p80
 1.Introduction / p81
 2.Materials and methods / p83
 3.Results / p85
 4.Discussion / p96
 5.Summary / p100
 6.References / p101
GENERAL DISCUSSION(CONCLUSION) / p107
REFERRENCE PUBLICATIONS / p109
RELATED PUBLICATIONS / p111
ACKNOWLEDGEMENTS / p113
SelfDOI
language
eng
nii type
Thesis or Dissertation
HU type
Doctoral Theses
DCMI type
text
format
application/pdf
text version
ETD
rights
Copyright(c) by Author
grantid
乙第2657号
degreeGrantor
広島大学(Hiroshima University)
degreename Ja
博士(工学)
degreename En
Engineering
degreelevel
doctoral
date of granted
1995-02-09
department
Graduate School of Engineering