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ID 51072
file
creator
Mekawy, Ahmad Mohammad M.
Assaha, Dekoum V. M.
subject
Gene expression
Metallothioneins
OsMT-3a
Salinity stress
Stress combination
abstract
Metallothioneins (MT) are primarily involved in metal chelation. Recent studies have shown that MT proteins are also involved in the responses of plants to various environmental stresses. The rice metallothionein-like gene OsMT-3a is upregulated by salinity and various abiotic stressors. A DNA construct containing the complete OsMT-3a coding sequence cloned downstream to the CaMV35S promoter was transformed into Arabidopsis and homozygous single-copy transgenic lines were produced. Compared to wild-type plants, transgenic plants showed substantially increased salinity tolerance (NaCl), drought tolerance (PEG), and heavy metal tolerance (CdCl2) as individual stresses, as well as different combinations of these stresses. Relevantly, under unstressed control conditions, vegetative growth of transgenic plants was also improved. The shoot Na+ concentration and hydrogen peroxide in transgenic plants were lower than those in wild-type plants. OsMT-3a-overexpressing Arabidopsis lines accumulated higher levels of Cd2+ in both shoots and roots following CdCl2 treatment. In the transgenic MT-3a lines, increased activity of two major antioxidant enzymes, catalase and ascorbate peroxidase, was observed. Thus, rice OsMT-3a is a valuable target gene for plant genetic improvement against multiple abiotic stresses.
description
This research was supported by JSPS KAKENHI Grant Numbers 25850041, 15KK0283, 16K07643 to AU and Ministry of Higher Education and Scientific Research in Egypt to AMMM.
journal title
Journal of Plant Research
volume
Volume 133
start page
429
end page
440
date of issued
2020-04-06
publisher
Springer
issn
0918-9440
1618-0860
publisher doi
language
eng
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
format
application/pdf
text version
author
rights
This is a post-peer-review, pre-copyedit version of an article published in Journal of Plant Research. The final authenticated version is available online at: https://doi.org/10.1007/s10265-020-01187-y
This is not the published version. Please cite only the published version. この論文は出版社版ではありません。引用の際には出版社版をご確認、ご利用ください。
relation url
department
Graduate School of Integrated Sciences for Life