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ID 29244
file
creator
Satoh, Tetsuya
Shinoda, Yasuharu
Tokonami, Shiho
Hirota, Ryuichi
subject
Bioluminescence assay
ATP amplification
Continuous-flow system
PDMS chip
NDC
Chemistry
abstract
In this study, we constructed a novel microfluidic device for continuous-flow ATP amplification, using the SU-8:PDMS method. Sepharose beads immobilized with adenylate kinase and pyruvate kinase was packed into a microfluidic chamber to form lamination layer. Dry film type SU-8 was suitable to form a very thick mold for beads column reactor and its dam structure. A good correlation between amplified luminescence and initial ATP concentration was observed in this system. The gradient of amplification when performing six cycles of continuous-flow ATP amplification was 1.72(N).
journal title
Sensors and Actuators B: Chemical
volume
Volume 142
issue
Issue 1
start page
118
end page
122
date of issued
2009-10-12
publisher
Elsevier Science Sa
issn
0925-4005
ncid
publisher doi
language
eng
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
format
application/pdf
text version
author
rights
Copyright (c) 2009 Elsevier B.V.
relation url
department
Research Center for Nanodevices and Systems