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ID 29231
file
creator
Goto, Hitoshi
Ahmed, Abo Bakr F
subject
Bioluminescence
Endotoxin
Firefly luciferase
Limulus amebocyte lysate
NDC
Biology
abstract
The Limulus reaction is an application of the defense mechanism of horseshoe crab for endotoxin detection. Endotoxin is a component of the cell wall in the outer membrane of gram-negative bacteria, and causes fever or shock when it enters the human blood stream. For endotoxin detection, gel formation or turbidity of the coagulation factor chromogen or fluorescence-modified peptide is used. However, these conventional methods have problems with regard to their measurement time or sensitivity. We recently obtained a mutant firefly luciferase that has a luminescence intensity over 10-fold higher than that of the wild type. Therefore, we developed a new endotoxin detection method that combines the Limulus reaction and bioluminescence using mutant luciferase. The new method detects 0.0005 EU/ml of endotoxin within 15 min.
journal title
Analytical Biochemistry
volume
Volume 397
issue
Issue 2
start page
152
end page
155
date of issued
2010-02-15
publisher
Academic Press Inc
Elsevier Science
issn
0003-2697
ncid
publisher doi
language
eng
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
format
application/pdf
text version
author
rights
Copyright (c) 2009 Elsevier Inc. All rights reserved.
relation url
department
Research Center for Nanodevices and Systems