このエントリーをはてなブックマークに追加
ID 28856
file
creator
Satoh, Tetsuya
Tsuruta, Kosuke
Shinoda, Yasuharu
Hirota, Ryuichi
subject
ATP amplification
Reciprocating-flow system
Bioluminescence assay
NDC
Biology
abstract
We constructed a novel ATP amplification reactor using a reciprocating-flow system to increase the number of ATP amplification cycles without an increase in backpressure. We previously reported a continuous-flow ATIP amplification system that effectively and quantitatively amplified ATP and increased the sensitivity of a quantitative bioluminescence assay. However, it was difficult to increase the number of amplification cycles due to backpressure in the system. Because addition of immobilized adenylate kinase (ADK) and pyruvate kinase (PK) columns increased backpressure, the maximum number of ATP amplification cycles within Column durability was only 4. In this Study, ATP amplification was performed using a reciprocating-flow system, and 10 cycles of ATP amplification could be achieved without an increase in backpressure. As a result, ATP was amplified more than 100-fold after 10 cycles of reciprocating flow. The gradient of ATP amplification was approximately 1.76(N). The backpressure oil the columns was 0.03 MPa in 1-10 ATIP amplification cycles, and no increases in backpressure were observed.
journal title
Analytical Biochemistry
volume
Volume 395
issue
Issue 2
start page
161
end page
165
date of issued
2009-12-15
publisher
Academic Press INC
Elsevier Science
issn
0003-2697
ncid
publisher doi
language
eng
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
format
application/pdf
text version
author
rights
Copyright (c) 2009 Elsevier Inc.
relation url
department
Research Center for Nanodevices and Systems