Trans-differentiation of a Duodenal Phenotype on Duodenal Transplantation of Different Normal Tissues in F344 Rats
Use this link to cite this item : https://ir.lib.hiroshima-u.ac.jp/00031592
ID | 31592 |
file | |
creator |
Kominami, Yoko
Gon, Rina
Hayashi, Masaomi
Nishiki, Masayo
Sasaki, Atsushi
Shiraishi, Miho
Ueda, Shinobu
Ochiya, Takahiro
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subject | Transplantation
Hetero-tissues
Duodenum
F344 GFP Rats
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NDC |
Medical sciences
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abstract | The mechanisms regulating stem cell differentiation and self-renewal are largely unknown. Our ultimate goal is to be able to regulate somatic stem cell differentiation and proliferation, In the present study the ability of trans-differentiation was studied when different normal tissue types were transplanted into the duodenum in rats. Pieces of ear (skin), bladder, trachea, diaphragm, pyloric gland. and forestomach from 8-week old GFP-F344 rats were transplanted into the duodenum of F344 rats. Goblet cells with alcian-blue PAS positive mucin and brash border with alkaline phosphatase (ALP) activity appeared in tissues implanted into the duodenum. In addition, GFP-positive duodenal mucosa was observed in all cases by immunohistochemical staining. Moreover, the GFP-positive cells were found to carry the GFP transgene by PCR analysis. indicating that the bladder, trachea. ear (skin), diaphragm, pyloric gland, and farestomach tissues showed a multipotential ability for differentiation. These results indicated that stem cells within tissues have a multipotential ability, trans-differentiating into different organs when transplanted into different environments.
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journal title |
Hiroshima Journal of Medical Sciences
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volume | Volume 60
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issue | Issue 1
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start page | 1
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end page | 6
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date of issued | 2011-03
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publisher | Hiroshima University Medical Press
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issn | 0018-2052
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ncid | |
language |
eng
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nii type |
Departmental Bulletin Paper
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HU type |
Departmental Bulletin Papers
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DCMI type | text
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format | application/pdf
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text version | publisher
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department |
Graduate School of Biomedical Science
Research Institute for Radiation Biology and Medicine
Natural Science Center for Basic Research and Development
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他の一覧 |