Induction of Cataract in Methylnitrosourea Treated Fischer (F344) Rats
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Aggregation of βH-crystallin
Cataracts were observed in female F344 rats who intravenously received methylnitrosourea (MNU), a potent carcinogen for multiple organs, in one dose of 50 mg/kg body weight. Induction of cataract 40 weeks after MNU treatment was 41 % whereas no cataract was observed in control rats. The aggregation of βH-crystallin fraction by MNU was studied in vitro. HPLC pattern ofβH-crystallin changed when lens protein was incubated for 24 hrs with MNU. HPLC patterns indicated that MNU induced high molecular weight aggregates ofβH-crystallin. This study conveys some indication about the direct interaction of MNU with lens protein in cataract formation.
This work was supported in part by Grant-in-Aid for Cancer Research from the Ministry of Education, Science and Culture, Japan.
Hiroshima Journal of Medical Sciences
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Hiroshima University Medical Press
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Research Institute for Radiation Biology and Medicine
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