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ID 37950
file
creator
Kumazaki, Tsutomu
subject
Cellular aging
Fibroblast
Fibronectin
In situ hybridization
NDC
Medical sciences
abstract
Several changes in the functional characteristics of fibronectin (FN) have been noted as cells become senescent in culture. In a previous report we showed that the steady state level of FN mRNA increases significantly during the process of in vitro cellular aging in a fibroblast strain. Because a phenomenon observed in one cell strain may not be the case in other cell strains, we extended the previous study and confirmed that this is a common phenomenon in at least two fibroblast strains of different origin. The greatest change in the proportion of cells expressing high levels of FN occurs near the end of a culture's proliferative potential. The proportion of cells unable to synthesize DNA follows a similar pattern. We also found that increasing cell size correlates closely with higher levels of FN expression. Thus, there is a clear correlation between increased FN mRNA content and in vitro cellular senescence. In order to see if this phenomenon could also be observed in cells aged in vivo, we analyzed cells aged in vivo. We found that fibroblasts from donors of higher age show lower labeling index, express a higher level of FN and come to have a larger cell area, similar to cells aged in vitro. This strongly suggests that a fibroblast in vivo ages with aging of the individual in like manner to that observed in in vitro aging cells, that is, by exhausting division potential. This supports studies using in vitro aging cells as a model for cellular aging in vivo.
description
This work was supported in part by grants-in-aid from Ministry of Education, Science and Culture of Japan ( # 02771747 and # 04836018).
journal title
Hiroshima Journal of Medical Sciences
volume
Volume 41
issue
Issue 4
start page
101
end page
104
date of issued
1992-12
publisher
Hiroshima University Medical Press
issn
0018-2052
ncid
pubmed id
language
eng
nii type
Departmental Bulletin Paper
HU type
Departmental Bulletin Papers
DCMI type
text
format
application/pdf
text version
publisher
department
Research Institute for Radiation Biology and Medicine
Graduate School of Biomedical Science
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