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ID 31947
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ヒト腫瘍における多剤耐性遺伝子MDR1の発現に関する研究
creator
Kim, Rung-sa
subject
Multidrug resistance gene (MDR1)
P-glycoprotein
Adriamycin resistance
Human tumors
NDC
Medical sciences
abstract
The expression of MDR1 gene was investigated in human solid tumors with respect to adriamycin resistance. Forty fresh human surgical specimens were analyzed by RNA dot blot assay for their expression of the human MDR1 gene and by immunohistological staining using a monoclonal antibody against P-glycoprotein (MDR1 gene product). The MDR1 mRNA level was increased in 11 cases of 40 cancer patients, including three rectal cancers, two breast cancers, two gastric cancers, one colon cancer, one renal cell carcinoma, one gall bladder cancer and one malignant lymphoma of stomach. However, considerable variation of the MDR1 mRNA level was noted among cancers of a specific type. Immunohistochemical studies with the monoclonal antibody were shown to be positive in 18 tumors. In all tumors tested, the MDR1 mRNA level and the immunohistochemical analysis showed a significant correlation. However, two of five tumors which resisted adriamycin treatment were found to be negative in MDR1 transcript, but positive in immunohistological analysis. These results indicate that immunohistochemical analysis would be more sensitive for detecting P-glycoprotein-expression, and that resistance to adriamycin, being multifactorial, can be associated at least, in part with the increased amount of MDR1 gene product.
language
eng
nii type
Thesis or Dissertation
HU type
Doctoral Theses
DCMI type
text
format
application/pdf
text version
ETD
rights
Copyright(c) by Author
relation references
Hiroshima J. Med. Sci. Vol.39, No.3, 71~77, September, 1990
grantid
乙第2062号
degreeGrantor
広島大学(Hiroshima University)
degreename Ja
博士(医学)
degreename En
Medicine
degreelevel
doctoral
date of granted
1990-11-22
department
Research Institute for Radiation Biology and Medicine
Graduate School of Biomedical Science