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ID 31111
file
creator
Ochiya, Takahiro
Ueda, Shinobu
Kominami, Yoko
Gon, Rina
Nishiki, Masayo
Hayashi, Masaomi
Sasaki, Atsushi
Shiraishi, Miho
Kashimoto, Naoki
Myojin, Yuki
subject
Differentiation
Hepatocyte
Transplantation
Stem cells
Liver
GFP
Transgenic rat
CCl4
NDC
Medical sciences
abstract
While organ-specific stem cells with roles in tissue injury repair have been documented, their pathogenic significance in diseases and the factors potentially responsible for their activation remain largely unclear. In the present study, heart, kidney, brain, and skin samples from F344 transgenic rats carrying the GFP gene were transplanted into normal F344 rat liver one day after an intraperitoneal injection (i.p.) of carbon tetrachloride (CCl4) to test their differentiation capacity. The transplantation was carried out by female donors to male recipients, and vice versa. One week after transplantation, GFP antigen-positive cells with phenotypic characteristics of hepatocytes were noted. After two weeks, their extent increased, and at 4 weeks, large areas of strongly GFP-stained cells developed. All recipient livers had GFP antigen-positive hepatocyte cells. PCR analysis coupled with laser capture micro-dissection (LCM) revealed those cells to contain GFP DNA. Thus, our results indicate that tissue stem cells have multipotential ability, differentiating into hepatocytes when transplanted into an injured liver.
journal title
Biochemical and Biophysical Research Communications
volume
Volume 354
issue
Issue 4
start page
841
end page
845
date of issued
2007-03-23
publisher
Elsevier
issn
0006-291X
ncid
publisher doi
language
eng
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
format
application/pdf
text version
author
rights
Copyright (c) 2007 Elsevier Inc. All rights reserved.
relation url
department
Research Institute for Radiation Biology and Medicine