Effect of Immunomodulatory Artificial Blood Exchange (IABX) on Guinea Pig-to-Rat Heart Xenografts
Use this link to cite this item : https://ir.lib.hiroshima-u.ac.jp/00037894
ID | 37894 |
file | |
creator |
Wada, Seishi
Murakami, Hironobu
Kajihara, Hiroki
Matsuura, Yuichiro
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subject | Xeno discordant transplantation
Hyperacute rejection
Artificial blood
Blood exchange
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NDC |
Medical sciences
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abstract | The aim of this study was to assess the effectiveness of pregraft immunomodulatory artificial blood exchange (IABX) in a guinea pig-to-rat xeno discordant heart transplantation, using an artificial blood (FC43 emulsion: The Green Cross, Japan) in exchange for a large volume of whole blood to remove humoral immune factors en bloc from the recipient rat. In the rats treated with IABX, rhythmic beating of the grafted heart was maintained for 2 hr, whereas the untreated heart beat lasted for only 15.2 ± 5.2 min (n=6). In the graft hearts treated with IABX, no pathologic changes such as multiple coronary thromboses due to hyperacute rejection (HAR) were observed. Humoral immune factors (natural-IgM titer, ACH50 and CH50 complement activities, platelet count, prothrombin time (PT), activated partial thromboplastin time (APTT) and fibrinogen serum concentration), which are thought to contribute to HAR, decreased significantly following the IABX treatment.
We conclude that IABX is an efficient method for prolonging the survival time of guinea pig heart xenografts by inhibiting thrombus formation in the xeno-graft heart. It was confirmed that IABX could remove recipient humoral immune factors en bloc. |
description | This study was supported by The Green Cross Co.,Ltd.
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journal title |
Hiroshima Journal of Medical Sciences
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volume | Volume 43
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issue | Issue 4
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start page | 153
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end page | 161
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date of issued | 1994-12
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publisher | Hiroshima University Medical Press
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issn | 0018-2052
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ncid | |
language |
eng
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nii type |
Departmental Bulletin Paper
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HU type |
Departmental Bulletin Papers
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DCMI type | text
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format | application/pdf
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text version | publisher
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department |
Graduate School of Biomedical Science
Graduate School of Health Science
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他の一覧 |