このエントリーをはてなブックマークに追加
ID 112
file
creator
Miyasako, Takanori
Kitayama, Shigeo
Dohi, Toshihiro
subject
IL-1
Voltage-dependent Ca2+ channel
P/Q-type Ca2+ channel
Catecholamine release
Ca2+
Adrenal chromaffin cells
NDC
Medical sciences
abstract
Effects of interleukin (IL) on intracellular free Ca2+ concentration ([Ca2+]i) rise and catecholamine (CA) release were examined in isolated, cultured bovine adrenal chromaffin cells. IL-1α and IL-1β inhibited the rise of [Ca2+]i and CA release induced by acetylcholine (ACh) and excess KCl both in normal and in Ca2+-sucrose medium. IL-1 receptor antagonist, IL-1RA by pretreatment blocked the inhibitory actions of IL-1. IL-1α reduced CA release induced by veratridine in normal medium but not in the presence of diltiazem. Analysis using specific blockers for voltage-operated Ca2+ channels revealed that IL-1α specifically inhibited the P/Q-type Ca2+ channel to reduce [Ca2+]i rise induced by excess KCl. IL-1 did not affect [Ca2+]i rise induced either by bradykinin or caffeine in Ca2+- deprivated medium or via activation of store-operated Ca2+ channel. The inhibitory effects of IL-1 were blocked by pretreatments of cells with herbimycin A, U0126 and PD 98054, but not with SB202190, SP 600125 or pertussis toxin (PTX), inhibited the induction of the inhibitory action of IL-1.These results demonstrated that IL-1 inhibits stimulation-evoked [Ca2+]i rise and CA release in chromaffin cells by blocking voltage-operated P/O-type Ca2+ channels. The inhibitory action of IL-1 may be mediated through tyrosine kinase and MEK/ERK pathways.
journal title
Biochimica et Biophysica Acta
volume
Volume 1673
issue
Issue 3
start page
160
end page
169
date of issued
2004-08-04
publisher
Elsevier B.V
issn
0304-4165
ncid
publisher doi
pubmed id
language
eng
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
format
application/pdf
text version
author
rights
Copyright (c) 2004 Elsevier B.V
relation is version of URL
http://dx.doi.org/10.1016/j.bbagen.2004.04.012
department
Graduate School of Biomedical Science