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ID 49832
file
creator
YAMAKIDO, Michio
YANAGIDA, Jitsuro
ISHIOKA, Sinichi
MATSUZAKA, Shigeru
HOZAWA, Soichiro
AKIYAMA, Mitoshi
KOBUKE, Kyoko
INAMIZU, Tsutomu
NISHIMOTO, Yukio
subject
Production of Interleukin 2
Human peripheral blood lymphocytes
Optimal condition
CTLL-2 microassay
NDC
Medical sciences
abstract
For the assay of the production of Interleukin 2 (IL-2) in human peripheral blood lymphocytes (human PBL), a study was made on the optimal condition for its culture.
1) The optimal condition for the production of IL-2 was considered to be incubation time of 24 hr, number of PBL of 1 x 106 cells/ml, and phytohemagglutinin-M (PHA-M) concentration of 1 — 5%
2) By one way mixed lymphocyte reaction (one way MLR), IL-2 activity similar in level to that by PHA-M stimulation could also be obtained. It was maximal at the 4th day of culture.
3) By PHA-P (0.06%) stimulation, IL-2 could also be produced similar to that by PHAM stimulation but it decreased in the order of Con A, PWM and PPD and hardly any production of IL-2 could be observed by LPS.
journal title
Hiroshima Journal of Medical Sciences
volume
Volume 34
issue
Issue 1
start page
43
end page
51
date of issued
1985-03
publisher
Hiroshima University School of Medicine
issn
0018-2052
ncid
pubmed id
language
eng
nii type
Departmental Bulletin Paper
HU type
Departmental Bulletin Papers
DCMI type
text
format
application/pdf
text version
publisher
department
Graduate School of Biomedical Science
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