Production of Interleukin 2 in Human Peripheral Blood Lymphocytes: Optimal Condition for its Culture
Use this link to cite this item : https://ir.lib.hiroshima-u.ac.jp/00049832
| ID | 49832 |
| file | |
| creator |
YAMAKIDO, Michio
YANAGIDA, Jitsuro
ISHIOKA, Sinichi
MATSUZAKA, Shigeru
HOZAWA, Soichiro
AKIYAMA, Mitoshi
KOBUKE, Kyoko
INAMIZU, Tsutomu
NISHIMOTO, Yukio
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| subject | Production of Interleukin 2
Human peripheral blood lymphocytes
Optimal condition
CTLL-2 microassay
|
| NDC |
Medical sciences
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| abstract | For the assay of the production of Interleukin 2 (IL-2) in human peripheral blood lymphocytes (human PBL), a study was made on the optimal condition for its culture.
1) The optimal condition for the production of IL-2 was considered to be incubation time of 24 hr, number of PBL of 1 x 106 cells/ml, and phytohemagglutinin-M (PHA-M) concentration of 1 — 5% 2) By one way mixed lymphocyte reaction (one way MLR), IL-2 activity similar in level to that by PHA-M stimulation could also be obtained. It was maximal at the 4th day of culture. 3) By PHA-P (0.06%) stimulation, IL-2 could also be produced similar to that by PHAM stimulation but it decreased in the order of Con A, PWM and PPD and hardly any production of IL-2 could be observed by LPS. |
| journal title |
Hiroshima Journal of Medical Sciences
|
| volume | Volume 34
|
| issue | Issue 1
|
| start page | 43
|
| end page | 51
|
| date of issued | 1985-03
|
| publisher | Hiroshima University School of Medicine
|
| issn | 0018-2052
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| ncid | |
| pubmed id | |
| language |
eng
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| nii type |
Departmental Bulletin Paper
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| HU type |
Departmental Bulletin Papers
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| DCMI type | text
|
| format | application/pdf
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| text version | publisher
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| department |
Graduate School of Biomedical Science
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| 他の一覧 |
