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ID 110
file
creator
Hashimoto, W.
Kitayama, S.
Kumagai, K.
Morioka, N.
Dohi, Toshihiro
subject
Dopamine
Levodopa
Monoamine Transporter
Norepinephrine
Serotonin
Cocaine
Parkinson's disease
NDC
Medical sciences
abstract
The present study investigated the effects of levodopa, a precursor of dopamine (DA) therapeutically used for the treatment of Parkinson's disease, on DA transport in the two different systems, COS-7 cells heterologously expressing rat monoamine transporter cDNA and in monoaminergic cell lines PC12 and SK-N-SH. Levodopa enhanced uptake of [3H]DA and [3H]norepinephrine (NE) but not [3H]serotonin in the transfected COS-7 cells in a concentration-dependent manner. On the other hand, in PC12 and SK-N-SH cells where NET is functionally expressed, levodopa enhanced [3H]DA and [3H]NE uptake at low concentrations and inhibited the uptake at higher concentrations. The effects of levodopa on catecholamine transporters in the opposite direction suggest a different mechanism at the intra- and extracellular sites in a levodopa transport-dependent and independent manner.
journal title
Life Sciences
volume
Volume 76
issue
Issue 14
start page
1603
end page
1612
date of issued
2005-02-18
publisher
Elsevier Inc.
issn
0024-3205
publisher doi
pubmed id
language
eng
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
format
application/pdf
text version
author
rights
Copyright (c) 2004 Elsevier Inc.
relation is version of URL
http://dx.doi.org/10.1016/j.lfs.2004.08.029
department
Graduate School of Biomedical Science