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ID 45842
file
creator
Gumay, Ainun Rahmasari
Bakri, Saekhol
Pudjonarko, Dwi
Suprihati
subject
Green tea epigallocatechin-3-gallate
spatial memory function
MDA
TNF- α
D- galactose
NDC
Medical sciences
abstract
Introduction : Neuroinflammatory process and oxidative stress play an important role in the mechanism of brain aging and neurodegenerative diseases such as Alzheimer. Epigallocatechin-3-gallate (EGCG) have antioxidant, anti-inflammatory, and neuroprotective effects. This study aims to prove the effect of green tea EGCG on spatial memory function, malondialdehyde (MDA), and TNF-α level in Balb/c mice induced by D-galactose. Method : An experimental study using ”post test only control group design”. The samples were 18 male Balb/c mices, aged 6-8 weeks, divided into 3 groups. Negative control group (N-C) was induced by subcutaneous injection of D-galactose (150 mg/kg) once daily for 6 weeks. EGCG-2 and EGCG-6 were induced by D-galactose and orally administered by 2 and 6 mg/kg EGCG once daily for 5 weeks. The indicator of examination were spatial memory function using morris water maze, MDA and TNF-α level using Elisa. One-way Anova, Kruskal-Wallis, and Pearson were used for statistical analysis. Results : Means of % escape latency time and path length in the EGCG-2 {42,02(SD=5,9);43,47(SD=5,97)%} and EGCG-6 {40,45(SD=6,5); 41,78(SD=6,77)%} were significantly higher than N-C {28,68(SD=9,15), p=0,013; 32,98(SD=7,75)%,p=0,04}. MDA level in the EGCG-2 {587,79(SD=76,04)ng/ml} was significantly smaller than N-C {722,64(SD=134,78)ng/ml,p=0,037}. TNF-α level in all groups was not different (p=0,786). There was a significant and strong correlation between MDA level and spatial memory function (r=-0.551; p=0,018). Conclusion : EGCG may improve spatial memory function and oxidative stress in mice induced dementia, but it may not improve the status of neuroinflammation.
journal title
Hiroshima Journal of Medical Sciences
volume
Volume 67
start page
41
end page
48
date of issued
2018-05
publisher
Hiroshima University Medical Press
issn
0018-2052
ncid
language
eng
nii type
Departmental Bulletin Paper
HU type
Departmental Bulletin Papers
DCMI type
text
format
application/pdf
text version
publisher
rights
Copyright (c) 2018 Hiroshima University Medical Press
department
Graduate School of Biomedical & Health Sciences
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