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ID 45836
file
creator
Agustina, Rizki Karunianti
Dieny, Fillah Fithra
Rustanti, Ninik
Anjani, Gemala
Afifah, Diana Nur
subject
tempeh gembus
hydrolysate
antioxidant activity
soluble protein
NDC
Medical sciences
abstract
Tempeh gembus is fermented soy-pulp product contains high protein and its bioactive peptide components has potential antioxidant activities. In this study bromelain enzyme was applied in tempeh gembus to break up peptides bond and released bioactive peptides and amino acids. The aim of this research was to analyse antioxidant activity and soluble protein of tempeh gembus hydrolysate. Experimental research with 4 bromelain enzymes were applied in tempeh gembus as 0 ppm, 5000 ppm, 8000 ppm, and 10000 ppm. Antioxidant activities were measured by ABTS and DPPH radicals test. Whil e soluble protein content was measured by Bradford test. In general, antioxidant activity of tempeh gembus was higher when measured by ABTS radical (63.14±1.16 – 92.85±2.28%) compared to DPPH radical (52.21±5.76 – 65.70±5.89%). Antioxidant activity of tempeh gembus hydrolysate in ABTS test differed significantly between treatment groups (p=0.001), but not on DPPH test (p=0.110). While soluble protein content of these protein hydrolysate were 0.58±0.05 – 0.78±0.11% and significantly differed between treatment groups (p=0.019). Antioxidant activity was significantly higher when measured by ABTS radical compared to DPPH radical with different
protein soluble content.
journal title
Hiroshima Journal of Medical Sciences
volume
Volume 67
start page
1
end page
7
date of issued
2018-05
publisher
Hiroshima University Medical Press
issn
0018-2052
ncid
language
eng
nii type
Departmental Bulletin Paper
HU type
Departmental Bulletin Papers
DCMI type
text
format
application/pdf
text version
publisher
rights
Copyright (c) 2018 Hiroshima University Medical Press
department
Graduate School of Biomedical & Health Sciences
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