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ID 20744
file
creator
Kawamoto, June-ko
Utani, Koh-ichi
subject
nucleolus
RNA FISH
nuclear structure and function
post-transcriptional gene regulation
c-myc
NDC
Biology
abstract
We used fluorescence in situ hybridization (FISH) to detect c-myc RNA subspecies in human COLO 320DM tumor cells. Although the FISH procedure removed the majority of RNAs from the nucleolus, c-myc RNA continued to be detected in both the nucleoplasm and nucleolus. This finding suggests stable association between c-myc RNA and the nucleolus. Nucleolar accumulation of c-myc RNA appeared to be temporally regulated by cell cycle progression. Hybridization with exon- and strand-specific RNA probes indicated that the non-protein coding exon 1 plays a novel role in determining the subnuclear localization of c-myc RNA. Antisense RNA targeting exon 2 localized only with nucleoplasmic foci, where it might interact with the sense strand. Thus, c-myc gene expression may be regulated by intranuclear localization of its RNA.
journal title
Biochemical and Biophysical Research Communications
volume
Volume 359
issue
Issue 3
start page
806
end page
810
date of issued
2007-08-03
publisher
Academic Press Inc Elsevier Science
issn
0006-291X
ncid
publisher doi
language
eng
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
format
application/pdf
text version
author
rights
Copyright (c) 2007 Elsevier Inc.
relation url
department
Graduate School of Biosphere Science