Regulation of c-myc through intranuclear localization of its RNA subspecies
BBRC_359-3_806.pdf 325 KB
nuclear structure and function
post-transcriptional gene regulation
We used fluorescence in situ hybridization (FISH) to detect c-myc RNA subspecies in human COLO 320DM tumor cells. Although the FISH procedure removed the majority of RNAs from the nucleolus, c-myc RNA continued to be detected in both the nucleoplasm and nucleolus. This finding suggests stable association between c-myc RNA and the nucleolus. Nucleolar accumulation of c-myc RNA appeared to be temporally regulated by cell cycle progression. Hybridization with exon- and strand-specific RNA probes indicated that the non-protein coding exon 1 plays a novel role in determining the subnuclear localization of c-myc RNA. Antisense RNA targeting exon 2 localized only with nucleoplasmic foci, where it might interact with the sense strand. Thus, c-myc gene expression may be regulated by intranuclear localization of its RNA.
Biochemical and Biophysical Research Communications
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Academic Press Inc Elsevier Science
Copyright (c) 2007 Elsevier Inc.
Graduate School of Biosphere Science