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ID 17107
file
creator
Elsaied, Hosam Easa
Kaneko, Ryo
subject
Bathymodiolus
Deep sea
Methanotroph
RuBisCO
Symbiont
NDC
Fishing industry. Fisheries
abstract
In our previous investigation on the genes of 1,5-bisphosphate carboxylase/oxygenase (RuBisCO; EC 4.1.1.39) in deep-sea chemoautotrophic and methanotrophic endosymbioses, the gene encoding the large subunit of RuBisCO form I (cbbL) had been detected in the gill of a mussel belonging to the genus Bathymodiolus from a western Pacific back-arc hydrothermal vent. This study further revealed the symbiont source of the RuBisCO cbbL gene along with the genes of 16S ribosomal RNA (16S rDNA), particulate methane monooxygenase (EC 1.14.13.25; pmoA) and ATP sulfurylase (EC 2.7.7.4; sopT). The 16S rDNA sequence analysis indicated that the mussel harbors a monospecific methanotrophic Gammaproteobacterium. This was confirmed by amplification and sequencing of the methanotrophic pmoA, while thiotrophic sopT was not amplified from the same symbiotic genome DNA. Fluorescence in situ hybridization demonstrated simultaneous occurrence of the symbiont-specific 16S rDNA, cbbL and pmoA, but not sopT, in the mussel gill. This is the first molecular and visual evidence for a methanotrophic bacterial endosymbiont that bears the RuBisCO cbbL gene relevant to autotrophic CO2 fixation.
journal title
Marine Biotechnology
volume
Volume 8
issue
Issue 5
start page
511
end page
520
date of issued
2006-10
publisher
Springer
issn
1436-2228
ncid
publisher doi
pubmed id
language
eng
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
format
application/pdf
text version
author
rights
Copyright (c) 2006 Springer-Verlag. "The original publication is available at www.springerlink.com"
relation is version of URL
http://dx.doi.org/10.1007/s10126-005-6135-5
department
Graduate School of Biosphere Science