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ID 20750
file
creator
Miyoshi, Miki
Fukushima, Yuji
Matsuda, Haruo
subject
chicken
interleukin-13 receptor
monoclonal antibody
NDC
Biology
abstract
Mammalian interleukin-13 (IL-13) is an important regulatory T2 cytokine secreted by activated T lymphocytes. The IL-13 receptor (IL-13R) has two different chains, IL-13Rα1 and IL-13Rα2. Although the chicken IL-13 gene is well characterized, little is known about IL-13Rs. We cloned a cDNA encoding the 380 amino acid pro-peptide of chicken IL-13Rα2 (chIL-13Rα2) and developed a monoclonal antibody (mAb), HU13-1, against it. The chIL-13Rα2 amino acid sequence showed 37-39% sequence identity with mammalian homologs. High levels of chIL-13Rα2 mRNA were expressed in liver, testis, ovary, brain, and lipopolysaccharide (LPS)-stimulated IN24 cells. HU13-1 specifically recognized recombinant chIL-13Rα2 in ELISAs, and western blots identified a 45-kDa glycoprotein or a 41-kDa non-glycosylated protein in LPSstimulated IN24 cell lysates. LPS induced a gradual increase in HU13-1-positive IN24 cells over 20 h. These results indicate that mAb HU13-1 recognizes native chIL-13Rα2 and will be valuable for further studies of chicken IL-13Rs.
journal title
Developmental and Comparative Immunology
volume
Volume 31
issue
Issue 4
start page
394
end page
406
date of issued
2007
publisher
Pergamon-Elsevier Science Ltd.
issn
0145-305X
ncid
publisher doi
language
eng
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
format
application/pdf
text version
author
rights
Copyright (c) 2006 Elsevier Ltd
relation url
department
Graduate School of Biosphere Science