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ID 17053
file
creator
Yamada, Kazuhiko
Nishida-Umehara, Chizuko
Ishijima, Junko
Murakami, Takahiro
Shibusawa, Mami
Tsuchiya, Kimiyuki
Matsuda, Yoichi
subject
W chromosome
repetitive DNA sequence
Japanese quail
guinea fowl
Neognathous birds
NDC
Biology
abstract
A novel family of repetitive DNA sequences were molecularly cloned from ApaI-digested genomic DNA of two Galliformes species, Japanese quail (Coturnix japonica) and guinea fowl (Numida meleagris), and characterized by chromosome in-situ hybridization and filter hybridization. Both the repeated sequence elements produced intensely painted signals on the W chromosomes, whereas they weakly hybridized to whole chromosomal regions as interspersed-type repetitive sequences. The repeated elements of the two species had high similarity of nucleotide sequences, and cross-hybridized to chromosomes of two other Galliformes species, chicken (Gallus gallus) and blue-breasted quail (Coturnix chinensis). The nucleotide sequences were conserved in three other orders of Neognathous birds, the Strigiformes, Gruiformes and Falconiformes, but not in Palaeognathous birds, the Struthioniformes and Tinamiformes, indicating that the repeated sequence elements were amplified on the W chromosomes in the lineage of Neognathous birds after the common ancestor diverged into the Palaeognathae and Neognathae. They are components of the W heterochromatin in Neognathous birds, and a good molecular cytogenetic marker for estimating the phylogenetic relationships and for clarifying the origin of the sex chromosome heterochromatin and the process of sex chromosome differentiation in birds.
journal title
Chromosome Research
volume
Volume 14
issue
Issue 6
start page
613
end page
627
date of issued
2006-09
publisher
Springer
issn
0967-3849
ncid
publisher doi
pubmed id
language
eng
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
format
application/pdf
text version
author
rights
Copyright (c) 2006 Springer-Verlag. "The original publication is available at www.springerlin.com"
relation is version of URL
http://dx.doi.org/10.1007/s10577-006-1071-4
department
Graduate School of Biosphere Science