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ID 48663
file
creator
Okabe, Ayami
Hiramatsu, Rui
Fujita, Youko
subject
luteinization
Migration
Granulosa cells
Semaphorin
Ovulation
abstract
Morphological changes are observed during the luteinization process of granulosa cells, however mechanisms and the roles are remained unclear. In the present study, we demonstrated that Sema3C is expressed in granulosa cells and then secreted to extracellular matrix accumulated within cumulus cell and granulosa cell layers. The expression is dependent on EGF-like factors (amphiregulin and neuregulin 1) and EGF receptor dependent manner. The knockdown of Sema3c not only significantly decreased the surface area of the granulosa cells, but also significantly suppressed the cell migration of granulosa cells cultured with EGF like factors. The phosphorylation of focal adhesion kinase (FAK) was dramatically suppressed by transfection with Sema3c siRNA, with an increasing level of phosphorylated ROCK, indicating that Sema3C regulated actin remodeling in a FAK signaling pathway-dependent manner. Moreover, the expression levels of genes involved in progesterone production, Star, Cyp11a1 were significantly decreased in Sema3c siRNA-transfected granulosa cells as compared with control siRNA transfection. From these results, we conclude that Sema3C is secreted and then accumulated within granulosa cell layers and cumulus cell layers. The FAK pathway is activated by Sema3C and induces cell migration and enlarges the area of granulosa cells. These morphological changes are required for luteinization (production of progesterone) of granulosa cells.
description
This work was supported, in part, by a grant-in-Aid for Scientific Research No. 24688028, and No. 21688019 (M. S.) from the Japan Society for the Promotion of Science (JSPS).
journal title
Journal of Mammalian Ova Research
volume
Volume 31
issue
Issue 1
start page
31
end page
39
date of issued
2014
publisher
Japan Society of Ova Research
日本卵子学会
issn
1347-5878
1341-7738
publisher doi
language
eng
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
format
application/pdf
text version
publisher
rights
© 2014 Japan Society of Ova Research
relation url
department
Graduate School of Biosphere Science