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ID 15064
file
creator
Yokoyama, Hiroshi
Okamoto, Michiyo
Yamamoto, Josuke
Hirata, Dai
subject
calcium
calcineurin
Swe1p
Yap1p
yeast
abstract
The Ca2+-activated pathways in Saccharomyces cerevisiae induce a delay in the onset of mitosis through the activation of Swe1p, a negative regulatory kinase that inhibits the Cdc28p/Clb complex. We isolated the YAP1 gene as a multicopy suppressor of calcium sensitivity owing to the loss of ZDS1, a negative regulator of SWE1 and CLN2 gene expression. YAP1 deletion on a zds1Δ background exacerbated the Ca2+-related phenotype. Yap1p was degraded in a calcineurin-dependent manner when cells were exposed to calcium. In yap1Δ cells, the expression level of the RPN4 gene encoding a transcription factor for the subunits of the ubiquitin - proteasome system was diminished. The deletion of YAP1 gene or RPN4 gene led to the accumulation of Swe1p and Cln2p. Yap1p was a substrate of calcineurin in vivo and in vitro. The calcineurin-mediated Yap1p degradation seems to be a long adaptive response that assures a G2 delay in response to a stress that causes the activation of the calcium signalling pathways.
journal title
EMBO Reports
volume
Volume 7
issue
Issue 5
start page
519
end page
524
date of issued
2006-05
publisher
Nature Publishing Group
issn
1469-221X
ncid
publisher doi
language
eng
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
text version
author
rights
Copyright (c) 2006 European Molecular Biology Organization
relation is version of URL
http://dx.doi.org/10.1038/sj.embor.7400647
department
Graduate School of Advanced Sciences of Matter