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ID 48968
file
creator
Hori, Akiko
Morand, Agathe
Ikebe, Chiho
Frith, David
Snijders, Ambrosius P.
subject
Bipolar spindle
Centriolar satellites
Centriole
Centrosome
Fission yeast
Spindle pole body (SPB)
Super resolution microscopy
WD repeats
abstract
The centrosome plays a pivotal role in a wide range of cellular processes and its dysfunction is causally linked to many human diseases including cancer and developmental and neurological disorders. This organelle contains more than one hundred components, and yet many of them remain uncharacterised. Here we identified a novel centrosome protein Wdr8, based upon the structural conservation of the fission yeast counterpart. We showed that Wdr8 constitutively localises to the centrosome and super resolution microscopy uncovered that this protein is enriched at the proximal end of the mother centriole. Furthermore, we identified hMsd1/SSX2IP, a conserved spindle anchoring protein, as one of Wdr8 interactors by mass spectrometry. Wdr8 formed a complex and partially colocalised with hMsd1/SSX2IP. Intriguingly, knockdown of Wdr8 or hMsd1/SSX2IP displayed very similar mitotic defects, in which spindle microtubules became shortened and misoriented. Indeed, Wdr8 depletion resulted in the reduced recruitment of hMsd1/SSX2IP to the mitotic centrosome, though the converse is not true. Together, we propose that the conserved Wdr8-hMsd1/SSX2IP complex plays a critical role in controlling proper spindle length and orientation.
description
T.T. and A.P.S were supported by Cancer Research UK.
Supplementary data related to this article can be found at http://dx.doi.org/10.1016/j.bbrc.2015.10.169.
journal title
Biochemical and Biophysical Research Communications
volume
Volume 468
issue
Issue 1-2
start page
39
end page
45
date of issued
2015-12
publisher
Elsevier
issn
0006-291X
1090-2104
publisher doi
pubmed id
language
eng
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
format
application/pdf
text version
publisher
rights
© 2015 The Francis Crick Insitute. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
relation url
department
Graduate School of Advanced Sciences of Matter