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ID 295
file
creator
Ueda, Hiroaki
NDC
Zoology
abstract
In order to determine the sex-determining mechanism in Bufo viridis, males were sex-reversed into females by castration and subsequent administration with estrogen, and mated with normal males to obtain the offspring. Of the 25 castrated and estrogen-injected males, 17 lived for more than 15 months. While seven of these toads had regenerated testes and normal BIDDER'S organs, the other ten had well-developed ovaries transformed from their BIDDER'S organs, although the oviducts were defective in five of them. By pituitary injection, four of the remaining five sex-reversed males laid 473,62,5368 and 7316 eggs. By insemination with sperm of regenerated testes, 822 eggs attained completion of metamorphosis. Among 784 offspring, there were 197 females and 587 (74.9%) males. These numbers seem to show that the male in Bufo viridis is heterogametic (XY) and YY toads produced from matings between sexreversed genetic males (XY) and normal males (XY) can survive and become males.
journal title
Scientific report of the Laboratory for Amphibian Biology
volume
Volume 10
start page
155
end page
164
date of issued
1990-03
publisher
Institute for Amphibian Biology, Hiroshima University
contributor
国立情報学研究所
date of created
2006-03-21
issn
0386-3166
ncid
SelfDOI
language
eng
nii type
Departmental Bulletin Paper
HU type
Departmental Bulletin Papers
DCMI type
text
format
application/pdf
text version
publisher
department
Graduate School of Science
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