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ID 46466
file
creator
Komaki, Shohei
Nozawa, Masafumi
Lin, Si-Min
Oumi, Shohei
subject
Amphibian
genetic diversity
island species
microsatellite markers
population genetics
NDC
Zoology
abstract
Buergeria japonica is a common frog species distributed throughout almost all islands in Ryukyu Archipelago. Because of their exceptionally wide distribution and higher physiological tolerance comparing to the other anurans, their demographic history and formation of distribution are intrinsic topics in the herpetological fauna of Ryukyu. Microsatellite marker is ideal genetic marker for such studies at inter- and intra-population level. We therefore developed microsatellite markers of B. japonica utilizing Ion PGM™ sequencing. As a result of the screening, we developed a total of 14 polymorphic markers. To test availabilities of these markers, we genotyped four island populations. The total number of alleles and expected hetelozygosities per locus ranged from 4 to 21 and 0.00 to 0.864, respectively. The phylogenetic relationship among the four populations based on the genetic distances of these markers was congruent with general divergence pattern of amphibians and reptiles in Ryukyu area. These markers developed in this study are considered to be useful for future studies about phylogeography and demography of this species.
description
This work was supported by a Grant-in-Aid for JSPS Fellows (No. 25-5065).
journal title
Genes & Genetic Systems
volume
Volume 89
issue
Issue 1
start page
35
end page
39
date of issued
2014-02
publisher
日本遺伝学会
issn
1341-7568
1880-5779
ncid
publisher doi
naid
language
eng
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
format
application/pdf
text version
publisher
rights
© 2014, Genetics Society of Japan
Publisher permitted to deposit the final published paper on this repository. 発行元の許可を得て登録しています.
relation url
department
Graduate School of Science