We study non-orientable Seifert surfaces for knots in the 3-sphere, and examine their boundary slopes. In particular, it is shown that for a crosscap number two knot, there are at most two slopes which can be the boundary slope of its minimal genus non-orientable Seifert surface, and an infinite family of knots with two such slopes will be described. Also, we discuss the existence of essential non-orientable Seifert surfaces for knots.