Characterization and Mutational Analysis of a Two-Polypeptide Bacteriocin Produced by Citrus Iyo-Derived Lactobacillus brevis 174A
Use this link to cite this item : https://ir.lib.hiroshima-u.ac.jp/00046814
ID | 46814 |
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creator |
Miyauchi, Rumi
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subject | bacteriocin
Lactobacillus brevis
actic acid bacteria
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abstract | In the present study, we isolated a lactic acid bacterium (LAB) from a citrus iyo fruit and identified it as Lactobacillus brevis. This plant-derived LAB strain, designated 174A, produces bacteriocin consisting of two polypeptides designated brevicin 174A-β and 174A-γ. Although each polypeptide itself displays antibacterial activity, the ability is enhanced 100 fold by mixing both polypeptides at a 1 : 1 ratio. Significantly, brevicin 174A inhibits even the growth of several pathogenic bacteria that are more resistant to a lantibiotic bacteriocin, nisin A, which is commonly utilized as a preservative added to foodstuffs. Structural analysis of the 174A bacteriocin using a program that predicts secondary structure suggests that both component polypeptides have a positively charged N-terminal region, as well as two cysteine residues in both the N- and C-terminals. Judging from a mutational analysis of the antibacterial polypeptides, these unique amino acid sequences of 174A-β might be important for the expression of the synergistic activity that occurs in the presence of the two polypeptides combined.
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journal title |
Biological and Pharmaceutical Bulletin
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volume | Volume 38
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issue | Issue 12
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start page | 1902
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end page | 1909
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date of issued | 2015-12-01
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publisher | The Pharmaceutical Society of Japan
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issn | 0918-6158
1347-5215
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publisher doi | |
pubmed id | |
language |
eng
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nii type |
Journal Article
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HU type |
Journal Articles
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DCMI type | text
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format | application/pdf
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text version | publisher
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rights | Copyright (c) 2015 The Pharmaceutical Society of Japan
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relation url | |
department |
Graduate School of Biomedical & Health Sciences
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