The Sarin-like Organophosphorus Agent bis(isopropyl methyl)phosphonate Induces Apoptotic Cell Death and COX-2 Expression in SK-N-SH Cells
Use this link to cite this item : https://ir.lib.hiroshima-u.ac.jp/00039746
ID | 39746 |
file | |
creator |
Arima, Yosuke
Yoshimoto, Kanji
Makita, Ryosuke
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subject | Sarin
Organophosphates
Apoptosis
COX-2
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NDC |
Medical sciences
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abstract | Organophosphorus compounds, such as sarin, are highly toxic nerve agents that inhibit acetylcholinesterase (AChE), but not cholinesterase, via multiple mechanisms. Recent studies have shown that organophosphorus compounds increase cyclooxygenase-2 (COX-2) expression and induce neurotoxicity. In this study, we examined the toxicity of the sarin-like organophosphorus agent bis(isopropyl methyl)phosphonate (BIMP) and the effects of BIMP on COX-2 expression in SK-N-SH human neuroblastoma cells. Exposure to BIMP changed cell morphology and induced caspase-dependent apoptotic cell death accompanied by cleavage of caspase 3, caspase 9, and poly (ADP-ribose) polymerase (PARP). It also increased COX-2 expression, while pretreatment with a COX inhibitor, ibuprofen, decreased BIMP-dependent cell death and COX-2 expression in SK-N-SH cells. Thus, our findings suggest that BIMP induces apoptotic cell death and upregulates COX-2 expression.
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journal title |
Hiroshima Journal of Medical Sciences
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volume | Volume 65
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issue | Issue 1
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start page | 1
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end page | 8
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date of issued | 2016-03
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publisher | Hiroshima University Medical Press
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issn | 0018-2052
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ncid | |
language |
eng
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nii type |
Departmental Bulletin Paper
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HU type |
Departmental Bulletin Papers
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DCMI type | text
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format | application/pdf
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text version | publisher
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rights | Copyright (c) 2016 Hiroshima University Medical Press
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department |
Graduate School of Biomedical & Health Sciences
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他の一覧 |