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ID 17064
file
creator
Sasabe, Eri
Miyazaki, Kazuko
Fujimotoa, Katsumi
Kato, Yukio
subject
Dec1
Stra13
Bhlhb2
DEC2
mesenchymal stem cell
osteogenesis
adipogenesis
NDC
Medical sciences
abstract
We recently reported that forced expression of basic helix-loop-helix transcription factor Dec1 accelerated chondrogenic differentiation of mesenchymal stem cells (MSC) in pellet cultures (M. Shen, E. Yoshida, W. Yan, T. Kawamoto, K. Suardita, Y. Koyano, K. Fujimoto, M. Noshiro and Y. Kato, 2002. J. Biol. Chem. 277:50112-50120). Since MSC have multilineage differentiation potential, we investigated the roles of Dec1 in osteogenic and adipogenic differentiation of human bone marrow-derived MSC. After osteogenic induction of MSC in medium containing dexamethasone, β- glycerophosphate, and ascorbic acid, Dec1 expression gradually increased from day 5 to day 14, while expression levels of Dec1 mRNA markedly decreased on days 3 and 7 after adipogenic induction. Infection with adenovirus expressing Dec1 raised mRNA levels of several bone characteristic molecules such as osteopontin, PTH receptor and alkaline phosphatase, even in the absence of the osteogenic-induction medium, although it had little effect on Runx2 expression or calcification. In the osteogenic-induction medium, Dec1 overexpression enhanced the expression of osteopontin and alkaline phosphatase and induced matrix calcification. Knockdown of Dec1 with siRNA suppressed the expression of osteoblastic phenotype by the induced MSC. Using MSC cultures, we also confirmed that forced expression of Dec1 suppressed adipogenic differentiation. These findings suggest that Dec1 modulates osteogenic differentiation of MSC by inducing the expression of several, but not all, bone-related genes.
journal title
European Journal of Cell Biology
volume
Volume 85
issue
Issue 5
start page
423
end page
431
date of issued
2006-03-03
publisher
Elsevier
issn
0171-9335
ncid
publisher doi
pubmed id
language
eng
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
format
application/pdf
text version
author
rights
Copyright (c) 2006 Elsevier GmbH.
relation url
department
Graduate School of Biomedical Science