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ID 20948
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title alternative
Basic Studies of Anti-Recipient Alloantiserum for Immunosuppressive Properties
creator
NDC
Medical sciences
abstract
BALB/Cの皮膚をC3H/Heマウスに移植し,1×10<7>ヶのBALB/C splenocyteを1週間隔で4回腹腔内注射して免疫することによりC3H/He anti-BALB/C alloantiserumを作成した。このalloantiserumはBALB/C cellに対して特異的ににcytotoxic (×128)であった。C3H/He anti-BALB/C alloantiserum (anti-recipient alloantiserum)がBALB/Cマウスに移植されたC3H/Heの皮膚の生者を延長さす能力をもつか否かを検討した。BALB/C recipientにC3H/Heの皮膚を移植する前後3回,C3H/He anti-BALB/C alloantiserumを腹腔内授与すると,1回投与量が50-300μlの量では移植皮膚の生着を延長さすことができた。in vitro studyにおいて,MLRはこのalloantiserumの存在下で特異的に抑制された。CMLも同じようにBALB/C cellをこのalloantiserumによってpriming phaseで前処置することにより特異的に抑制された。このalloantiserumによる処置はthird party alloantigenに対する反応にはintactであった。このデータから次のことが示唆される。つまり,このanti-recipient alloantiserumはskin allograftの生者に対して生者延長効果を示し,MLRとCMLに対してはspecific inhibitionを示した。これは,このalloantiserumがimmune responseのafferent archのblockをすることを示す。
abstract
C3H/He anti-BALB/c alloantiserum was prepared by BALB/C skin grafting to C3H/He mice followed by a total of four injections of 1 x 10<7> BALB/C splenocytes on C3H/He mice at one week interval. This alloantiserum was specifically cytotoxic (x 128) to BALB/C cells. C3H/He anti-BALB/C alloantiserum (anti-recipient alloantiserum) was tested to determine whether it possessed the ability to enhance C3H/He skin graft on BALB/C mice. Injection of C3H/He anti-BALB/C alloantiserum three times to BALB/C rcipients before and after C3H/He skin grafting at a single dose between 50 to 300 μl was effective in enhancing the skin graft survival. In in vitro study, mixed lymphocyte culture response (MLR) was specifically inhibited in the presence of this alloantiserum. Cell-mediated lympholysis (CML) was also specifically inhibited by pretreatment of BALB/C effector cells at the priming with this alloantiserum. This alloantiserum treatment left intact the response to third party alloantigens. The data suggested that this alloantiserum possessed in enhancing effect on skin graft survival, specifically inhibited MLR and CML, and consequently blocked the afferent arch of immune response.
description
広島大学医学雑誌, 32(3), 635-649, 昭59-6月(1984)
volume
Volume 32
issue
Issue 3
start page
635
end page
649
issn
0018-2087
ncid
language
jpn
nii type
Thesis or Dissertation
HU type
Doctoral Theses
DCMI type
text
format
application/pdf
rights
Copyright(c) by Author
grantid
乙第1218号
degreeGrantor
広島大学(Hiroshima University)
degreename Ja
博士(医学)
degreename En
Medicine
degreelevel
doctoral
date of granted
1984-09-27
department
Graduate School of Biomedical Science