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ID 40406
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title alternative
Development of a simple, hand made fluorescence microscope and a proposal to its practical use for the high school biology
creator
Saiga, Daisuke
Maga, Ayane
Kinutani, Kazuko
Fukuda, Mika
NDC
Education
abstract
In the present study, a simple hand made fluorescence microscope was developed. Four UV-LED lamps (NS360L-5RLO, Nitride Semiconductors Co., Ltd.) were used for the ultraviolet irradiation device. Some problems of a sample device have been improved and that has been completed. As the result, the nuclear fluorescence of the epidermal tissue of Tradescantia ohiensis stained by DAPI (4',6-diamidino-2-phenylindole) were observed.

This low cost simple fluorescence microscope was very effective for the student’s activities in the high school biology classes. In this paper, authors proposed an example of experiment which analyze relative DNA content value of nucleus in mitotic cell cycle by using this fluorescence microscope.
description
本研究の一部は,科学研究費基盤研究A(25242015)および科学研究費基盤研究C(26350235)の助成を受けて行ったものである。
journal title
Hiroshima journal of school education
volume
Volume 22
start page
143
end page
148
date of issued
2016-03-22
publisher
広島大学大学院教育学研究科附属教育実践総合センター
issn
1341-111X
ncid
language
jpn
nii type
Departmental Bulletin Paper
HU type
Departmental Bulletin Papers
DCMI type
text
format
application/pdf
text version
publisher
department
Graduate School of Education
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