このエントリーをはてなブックマークに追加
ID 25967
file
creator
Satoh, Tetsuya
Shinoda, Yasuharu
Alexandrov, Maxym
subject
Bioluminescence assay
ATP amplification
Continuous-flow system
NDC
Biology
abstract
We constructed a novel ATP amplification reactor using a continuous-flow system, and this allowed us to increase the sensitivity of quantitative bioluminescence assay by controlling the number of ATP amplification cycles. We previously developed a bioluminescence assay coupled with ATP amplification using a batch system. However, it was difficult to control the number of amplification cycles. In this study, ATP amplification was performed using a continuous-flow system, and significant linear correlations between amplified luminescence and initial ATP concentration were observed. When performing 4 cycles of continuous-flow ATP amplification, the gradient of amplification was 1.87N. Whereas lower quantifiable level was 500 pM without amplification, values as low as 50 pM ATP could be measured after amplification. Sensitivity thus increased 10-fold, with further improvements expected with additional amplification cycles. The continuous-flow system thus effectively increased the sensitivity of quantitative bioluminescence assay.
journal title
Analytical Biochemistry
volume
Volume 379
issue
Issue 1
start page
116
end page
120
date of issued
2008-08-01
publisher
Academic Press Inc.
Elsevier Science
issn
0003-2697
ncid
publisher doi
language
eng
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
format
application/pdf
text version
author
rights
Copyright (c) 2008 Elsevier Inc.
relation url
department
Graduate School of Advanced Sciences of Matter