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ID 39990
file
title alternative
On in vitro ovulation in the ovaries of spinous loach
creator
Utsunomiya, Yasuaki
Utsunomiya, Taeko
NDC
Zoology
abstract
(1) Some factors affected to ovulation from fragments of the ovaries of spinous 10aches immersed in frog pituitary suspension during the breeding season in vitro were researched.
(2) On density of hormone suspension,1/2 or 1/4 of frog pituitary per 1 cc of Ringer's solution were effective for ovulation.
(3) On treating duration by hormone suspension, it was the most effective for ovulation that fragments of the ovaries of spinous loaches were immersed in frog pituitary suspension between 3 or 5 hours and then were removed in pure Ringer's solution regulated value of pH.
(4) The most effective value of pH was 8.8 for ovulation.
(5) The value of pH in the ovary of the spinous loaches which were injected with frog pituitary suspension were between 6.6 or 6.8.
(6) Thus the eggs ovulated from the fragments in vitro cleaved normally after insemination.
journal title
Journal of the Faculty of Fisheries and Animal Husbandry, Hiroshima University
volume
Volume 5
issue
Issue 1
start page
179
end page
183
date of issued
1963-12-20
publisher
広島大学水畜産学部
issn
0440-8756
ncid
language
jpn
nii type
Departmental Bulletin Paper
HU type
Departmental Bulletin Papers
DCMI type
text
format
application/pdf
text version
publisher
department
Graduate School of Biosphere Science
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