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ID 37813
file
creator
Kurisu, Yoshihiro
Orihashi, Kazumasa
Kajihara, Hiroki
Matsuura, Yuichiro
subject
Cardiac allograft vasculopathy
Heart transplantation
Beraprost
Prostagrandin I2
NDC
Medical sciences
abstract
Intimal thickening and luminal narrowing of the coronary arteries are insidious complications of cardiac allograft. However, the pathogenesis of cardiac allograft vasculopathy (CAV) remains to be clarified. In this study, the protective effect of a prostacyclin analogue (beraprost sodium; BPS) on CAV was evaluated after heterotopic cardiac transplantation in rat. All recipients were treated with cyclosporine A (10 mg/kg/day intramuscularly). Eight rats received oral therapy with BPS of 50 μg/kg/day (BPS group) and another 8 rats received vehicle only (control group). All surviving cardiac grafts were removed on the 60th postoperative day and were examined to determine the severities of cellular rejection and CAV (>50 μm in diameter). Additionally, 6-keto-prostagrandin Fla and thromboxane B2 were compared between the two groups. There was no significant difference in the grading score for cellular rejection based on the ISHLT grading system (2.31 ± 0.75 vs 2.47 ± 0.65, p=0.81). Although the endothelial cells were preserved in both groups, a deposition of fibrin-like dense materials was recognized in the subendothelial layers of the control group, but not in the BPS group. Intimal thickening was inhibited significantly in the BPS group. The intimal ratio (intimal area/sectional area of artery) was significantly lower in the BPS group than in the control group (0.134 ± 0.03 vs 0.205 ± 0.047; p<0.01), without any difference in the medial ratio (medial area/sectional area of artery). a-actin positive smooth muscle cells (SMC) in intima were fewer in number in the BPS group than in the control group. The plasma thromboxane B2 level was significantly lower in the BPS group than in the control group (270 ± 116 pg/ml vs 585 ± 258 pg/mg; p<0.01). It was concluded that BPS suppressed CAV development after heterotopic allogenic cardiac transplantation in rats.
description
This study was presented at the 31th annual meeting of the Japan Society for Transplantation in September 1995, at the 36th annual meeting of the Japanese College of Angiology, November 1995, and at the 96th annual congress of the Japan Surgical Society in April 1996.
journal title
Hiroshima Journal of Medical Sciences
volume
Volume 46
issue
Issue 1
start page
11
end page
19
date of issued
1997-03
publisher
Hiroshima University Medical Press
issn
0018-2052
ncid
language
eng
nii type
Departmental Bulletin Paper
HU type
Departmental Bulletin Papers
DCMI type
text
format
application/pdf
text version
publisher
department
Graduate School of Biomedical Science
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