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ID 38142
file
creator
Yoshimoto, Kanji
Komura, Setsuo
Fujita, Tomio
subject
Brain dialysis
Dopamine
Serotonin
NDC
Medical sciences
abstract
The present study demonstrated that it is possible to measure the endogenous extracellular dopamine (DA) and its metabolite dihydroxyphenylacetic acid (DOPAC), endogenous extracellular 5-hydroxytryptamine (5HT), its metabolites 5-hydroxyindoleacetic acid (5HIAA), 5-hydroxytryptophol (5HTOL) and its precursor 5-hydroxytryptophan (5HTP) simultaneously from the striatum of freely moving rats using intracerebral dialysis combined with high performance liquid chromatography (HPLC) with electrochemical detector (ECD).

Basal extracellular levels of DA, DOPAC, 5HTP, 5HT, 5HIAA and 5HTOL in the striatum were estimated to be 18.89 ±. 2.23 (pg/25 μl/60 min), 7.25 ± 0.32 (ng/25 μl/60 min), 13.35 ± 3.01 (pg/25 μl/60 min), 5.32 ± 0.92 (pg/25 μl/60 min), 2.41 ± 0.08 (ng/25 μl/60 min) and 7.69 ± 0.45 (pg/25 μl/60 min), respectively. Pargyline (75 mg/kg i.p.) and tryptophan (100 mg/kg i.p.) increased the 5HT level in the striatum perfusates by 3-fold, whereas DOPAC and 5HIAA levels decreased by approximately 20% and 8%, respectively. In addition, DA and 5HTOL levels were not changed.

This method is suitable for monitoring dopaminergic and serotonergic activities dynamically.
journal title
Hiroshima Journal of Medical Sciences
volume
Volume 37
issue
Issue 1
start page
41
end page
43
date of issued
1988-03
publisher
Hiroshima University Medical Press
issn
0018-2052
ncid
pubmed id
language
eng
nii type
Departmental Bulletin Paper
HU type
Departmental Bulletin Papers
DCMI type
text
format
application/pdf
text version
publisher
department
Graduate School of Biomedical Science
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