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ID 37266
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title alternative
チャイニーズハムスター肺細胞由来グルコサミン要求変異株の細胞表面糖たんぱく及び細胞行動におけるN-アセチルグルコサミンの影響
creator
Onoda, Tetsuo
Nishigami, Kazuyoshi
NDC
Medical sciences
abstract
We have reported preliminary biological properties of mutant, G72-8, derived from Chinese hamster lung cells (Onoda, 1980 1)): the mutant is characterized by low adhesion to substratum, round shape, increase in spontaneous aggregation, and decrease in growth rate and these altered properties restored to normal by addition of GlcNAc. In this study, when L-fucose was used as radioactive precursor, the rate of L-fucose incorporation into macromolecules was markedly stimulated by addition of GlcNAc to the mutant. Lactoperoxidase-catalyzed iodiation of the cell surface shows that the high-molecular weight cell surface protein detected in the parent cells was reduced on the mutant and restored reversibly to normal by addition of GlcNAc. A random migration of the mutant cells also was reverted to the directional moving of the parent cells by addition of GlcNAc. Our results suggest that the carbohydrate moieties of cell surface proteins has an important role in biological cell behavior, including in adhesion and directionality in cell locomotion.
journal title
Hiroshima Journal of Medical Sciences
volume
Volume 30
issue
Issue 1
start page
29
end page
34
date of issued
1981-03-10
publisher
Hiroshima University School of Medicine
issn
0018-2052
ncid
language
eng
nii type
Departmental Bulletin Paper
HU type
Departmental Bulletin Papers
DCMI type
text
format
application/pdf
text version
publisher
department
Graduate School of Biomedical Science
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