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ID 38386
file
title alternative
シナプス膜への特異的セロトニン結合に及ぼすフィルターの影響
creator
Maeoka, Kunihiko
Sarai, Keisuke
subject
5-HT receptor binding assay
Filter
Low-affinity
High-affinity
NDC
Medical sciences
abstract
In the present study, the authors carried out some basic examinations of specific (3H)-5-Hydroxytryptamine (5-HT, serotonin) receptor binding assay. The most adequate incubation condition for (3H)-5-HT binding assay was at 0°C for 10 to 30 min. Scatchard plot of (3H)-5-HT binding using a Millpore filter and a Glass filter drew biphasic and monophasic, respectively. It is suggested that filter may affect on specific (3H)-5-HT binding. Therefore, the characteristics of filter should be taken into consideration in (3H)-5-HT binding assay.
journal title
Hiroshima Journal of Medical Sciences
volume
Volume 31
issue
Issue 2
start page
129
end page
131
date of issued
1982-06-10
publisher
Hiroshima University School of Medicine
issn
0018-2052
ncid
pubmed id
language
eng
nii type
Departmental Bulletin Paper
HU type
Departmental Bulletin Papers
DCMI type
text
format
application/pdf
text version
publisher
department
Graduate School of Biomedical Science
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