Characteristics of High-Molecular-Weight Hyaluronic Acid as a Brain-Derived Neurotrophic Factor Scaffold in Periodontal Tissue Regeneration
Use this link to cite this item : https://ir.lib.hiroshima-u.ac.jp/00032031
ID | 32031 |
file | |
creator |
Sakai, Noriyuki
Nagahara, Takayoshi
Kawaguchi, Hiroyuki
|
NDC |
Medical sciences
|
abstract | Brain-derived neurotrophic factor (BDNF), for which bovine collagen-derived atelocollagen is used as a scaffold, enhances periodontal tissue regeneration. However, a scaffold that does not contain unknown ingredients is preferable. Since the synthesized high-molecular-weight (HMW)-hyaluronic acid (HA) is safe and inexpensive, we evaluated the efficacy of HMW-HA as a BDNF scaffold. CD44, a major receptor of HA, was expressed in cultures of human periodontal ligament cells, and HMW-HA promoted the adhesion and proliferation of human periodontal ligament cells, although it did not influence the mRNA expression of bone (cementum)-related proteins. The in vitro release kinetics of BDNF from HMW-HA showed that BDNF release was sustained for 14 days. Subsequently, we examined the effect of BDNF/HMW-HA complex on periodontal tissue regeneration in dogs. A greater volume of newly formed alveolar bone and a longer newly formed cementum were observed in the BDNF/HMW-HA group than in the HMW-HA group, suggesting that HMW-HA assists the regenerative capacity of BDNF, although HMW-HA itself does not enhance periodontal tissue regeneration. Neither the poly (lactic-co-glycolic acid) group nor the BDNF/poly (lactic-co-glycolic acid) group enhanced periodontal tissue regeneration. In conclusion, HMW-HA is an adequate scaffold for the clinical application of BDNF.
|
journal title |
Tissue engineering. Part A.
|
volume | Volume 17
|
issue | Issue 7-8
|
start page | 955
|
end page | 967
|
date of issued | 2011
|
publisher | Mary Ann Liebert
|
issn | 1937-3341
|
ncid | |
publisher doi | |
language |
eng
|
nii type |
Journal Article
|
HU type |
Journal Articles
|
DCMI type | text
|
format | application/pdf
|
text version | publisher
|
rights | This is a copy of an article published in the Tissue engineering, Part A. (c) 2011 Mary Ann Liebert, Inc.; Tissue engineering, Part A. is available online at: http://online.liebertpub.com.
|
relation is version of URL | http://dx.doi.org/10.1089/ten.TEA.2010.0070
|
department |
Graduate School of Biomedical Science
|