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ID 30803
file
creator
Makihira, Seicho
Mine, Yuichi
Shuto, Takahiro
Iwata, Satoshi
Hosokawa, Ryuji
Kamoi, Kohei
Okazaki, Shota
Yamaguchi, Yu
subject
Titanium ion
Cell viability
TLR-2
TLR-4
Gingival epithelial cells
NDC
Medical sciences
abstract
Gingival epithelial-like cells (GE-1) were cultured and used to examine the cellular responses of gingival tissues to varying concentrations of titanium (Ti) ions. Titanium ions at concentrations of more than 13 ppm significantly decreased the viability of GE-1 cells and increased LDH release from the cells into the supernatant, but had no significant effect on their caspase 3 activity. These data suggest that a high concentration of Ti ions induced necrosis of the GE-1 cells. Titanium ions at a concentration of 5 ppm significantly increased the level of CCL2 mRNA expression in GE-1 cells exposed to lipopolysaccharide derived from Porphyromonas gingivalis in a synergistic manner. Moreover, the mRNA expression levels of TLR-4 and ICAM-1 in GE-1 cells loaded with Ti ions at 9 ppm were significantly enhanced as compared with those in GE-1 cells without Ti stimulation. We suggest that Ti ions are in part responsible for monocyte infiltration in the oral cavity by elevating the sensitivity of gingival epithelial cells to microorganisms. Taken together, these data indicate that Ti ions may be involved in cytotoxicity and inflammation at the interfaces of dental implants and gingival tissue.
journal title
Toxicology in Vitro
volume
Volume 24
issue
Issue 7
start page
1905
end page
1910
date of issued
2010-10
publisher
Pergamon Elsevier Science Ltd
issn
0887-2333
ncid
publisher doi
language
eng
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
format
application/pdf
text version
author
rights
Copyright (c) 2010 Elsevier Ltd
relation url
department
Graduate School of Biomedical Science