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ID 20323
file
creator
Nishimura, Haruki
Hamada, Taizo
Yamashiro, Hirofumi
Samaranayake, Lakshman P.
abstract
Effects of modified pellicles on Candida biofilm formation on acrylic surfaces. Abstract To examine the role of salivary or serum proteins, such as mucin, fibronectin and mannanbinding protein, the effect of modified pellicles on candidal biofilm formation was investigated in the present study. Supplementation of saliva with FN had no significant effect on biofilm activity. In contrast, the activity of the biofilm formed on either the mucin-coated or FN-coated acrylic was significantly less than that of the control (p<0.01). These results suggest that salivary mucin or FN alone does not facilitate the biofilm formation of Candida. In contrast, supplementation of serum with FN increased the biofilm activity of C. glabrata as compared with the control. In addition, pretreatment of serum with antiFN monoclonal antibody significantly reduced the biofilm activity of three isolates of Candida (p<0.01). These results, taken together, suggest that FN together with other serum components such as fibrinogen may facilitate Candida biofilm formation. Similarly, pretreatment of serum with antiMBP monoclonal antibody or Con-A seemed to significantly reduce the biofilm activity of some of the Candida species (p<0.05). Thus it seems that the cell surface mannnanin combination with MBP in serum play an important role in Candida biofilm formation. Our results taken together, suggested that not sole protein predominantly govern the biofilm formation of Candida, implying that candidal biofilm formation on acrylic surfaces is a complex phenomenon regulated by a multiplicity of proteins operating intra-orally.
journal title
Mycoses
volume
Volume 42
issue
Issue 1-2
start page
37
end page
40
date of issued
1999
publisher
Blackwell Publishing
issn
0019-3879
ncid
publisher doi
pubmed id
language
eng
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
format
application/pdf
text version
author
rights
Copyright (c) 1999 Blackwell Publishing. The definitive version is available at www.blackwell-synergy.com
relation url
department
Graduate School of Biomedical Science