Effects of modified pellicles on Candida biofilm formation on acrylic surfaces. Abstract To examine the role of salivary or serum proteins, such as mucin, fibronectin and mannanbinding protein, the effect of modified pellicles on candidal biofilm formation was investigated in the present study. Supplementation of saliva with FN had no significant effect on biofilm activity. In contrast, the activity of the biofilm formed on either the mucin-coated or FN-coated acrylic was significantly less than that of the control (p<0.01). These results suggest that salivary mucin or FN alone does not facilitate the biofilm formation of Candida. In contrast, supplementation of serum with FN increased the biofilm activity of C. glabrata as compared with the control. In addition, pretreatment of serum with antiFN monoclonal antibody significantly reduced the biofilm activity of three isolates of Candida (p<0.01). These results, taken together, suggest that FN together with other serum components such as fibrinogen may facilitate Candida biofilm formation. Similarly, pretreatment of serum with antiMBP monoclonal antibody or Con-A seemed to significantly reduce the biofilm activity of some of the Candida species (p<0.05). Thus it seems that the cell surface mannnanin combination with MBP in serum play an important role in Candida biofilm formation. Our results taken together, suggested that not sole protein predominantly govern the biofilm formation of Candida, implying that candidal biofilm formation on acrylic surfaces is a complex phenomenon regulated by a multiplicity of proteins operating intra-orally.