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ID 25954
file
creator
Hua, Ngoc-Phuc
Amel, Hamza-Chaffai
Vreeland, Russell H.
Isoda, Hiroko
NDC
Biology
abstract
A Gram-positive, endospore-forming, rod-shaped and moderately halophilic bacterium was isolated from a salt crust sample collected in Gharsa salt lake (Chott el Gharsa), Tunisia. The newly isolated bacterium designated SA-Vb1T was identified based on polyphasic taxonomy including genotypic, phenotypic and chemotaxonomic characterization. Strain SA-Vb1T was closely related to Virgibacillus marismortui and V. olivae with 16S rRNA gene sequence similarities of 99.7% and 99.4%, respectively. However, strain SA-Vb1T was distinguished from these two species on phenotypic characteristics and on the basis of DNA-DNA relatedness (29.4% and 5.1%, respectively). Genetic relationships between strain SA-Vb1T and the type species V. pantothenticus IAM 11061T and other type strains of the genus were 96-98% based on 16S rRNA gene and 18.3-22.3% based on DNA-DNA hybridization. Biochemical analysis resulted in determination of major fatty acids, iso-C15:0, anteiso-C15:0 and anteiso-C17:0 (33.3%, 29.2%, and 9.8%, respectively); phosphatidyl glycerol, diphosphatidyl glycerol, and phosphatidyl ethanolamine were the main polar lipids; and MK-7 the predominant menaquinone (~100%). The distinct characteristics demonstrated by these data show that strain SA-Vb1T represents properties of a novel species of the genus Virgibacillus. Accordingly, this isolate is proposed as Virgibacillus salarius sp. nov. The type strain is SA-Vb1T (=JCM 12946T =DSM 18441T).
journal title
International Journal of Systematic and Evolutionary Microbiology
volume
Volume 58
start page
2409
end page
2414
date of issued
2008
publisher
Society for General Microbiology
issn
1466-5026
ncid
publisher doi
language
eng
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
format
application/pdf
text version
author
rights
Copyright (c) 2008 International Union of Microbiological Societies
relation url
department
Graduate School of Biosphere Science