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ID 25654
file
creator
Nguyen, H.D.
Mushiake, Keiichi
Muroga, Kiyokuni
subject
Viral nervous necrosis (VNN)
Nodavirus
Striped jack
FAT
PCR
Pseudocaranx dentex
Brood stock
NDC
Zoology
abstract
Fluorescent antibody technique (FAT) and polymerase chain reaction (PCR) method were used to localize striped jack nervous necrosis virus (SJNNV, a nodavirus) in adult striped jack Pseudocaranx dentex. One group of brood stocks (N = 4) consisted of 13-yr-old spawners whose reproductive fluids were SJNNV-positive by the PCR test. The other group (N = 4) consisted of 4-yr-old fish which had not previously spawned whose reproductive fluids were negative by the PCR test. Positive FAT reactions using an anti-SJNNV rabbit serum were observed in the gonad, intestine, stomach, kidney, and liver of the 13-yr-old fish but not in the corresponding organs of the 4-yr-old fish. In neither group were the viral antigens detected in the spinal cord, brain, or retina tissues, the target organs of the virus in striped jack larvae. The FAT results were consistent with PCR results for the detection of the SJNNV coat protein gene. The present results suggest that SJNNV originates in various organs of striped jack spawners and is shed from the intestine and gonad, which results in contamination of eggs.
journal title
Diseases of Aquatic Organisms
volume
Volume 28
issue
Issue 2
start page
87
end page
91
date of issued
1997-02-13
publisher
Inter-Research
issn
0177-5103
1616-1580
ncid
publisher doi
language
eng
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
format
application/pdf
text version
publisher
rights
Copyright (c) 1997 Inter-Research.
relation url
department
Graduate School of Biosphere Science