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ID 25652
file
creator
Nguyen, H.D.
Muroga, Kiyokuni
subject
Viral nervous necrosis
Nodavirus
Striped jack
In vivo multiplication
NDC
Zoology
abstract
The progress of infection with SJNNV (nodavirus), the causative agent of viral nervous necrosis (VNN), was investigated by using naturally infected (acute and subacute groups) and experimentally infected striped jack larvae at different stages of infection. Although there were slight differences in the progress of infection among the 3 groups of fish examined, the general features of infection were quite similar. Necrosis and vacuolation of the nerve cells were first observed in the spinal cord, particularly in the area just above the swimbladder, later in the brain, and then in the retina. Mortalities occurred 1 to 2 d after the commencement of lytic degeneration of the cells, which resulted in heavy vacuolation in these nervous tissues. Virus antigens were detectable in the nervous tissues by the fluorescent antibody technique (FAT) when conspicuous vacuolation appeared in the cytoplasm. Virus particles were detectable by electron microscopy in concurrence with the appearance of heavy tissue vacuolation. These results indicate that SJNNV exhibits a tropism for nerve cells and its initial multiplication site is the spinal cord, from which the virus spreads to the brain and finally to the retina. Hyperplasia was observed in the skin of 1 naturally infected larval group (acute infection) and virus multiplication was observed in these affected epithelial cells. However, the role of skin as a portal of entry for SJNNV remains unclear.
journal title
Diseases of Aquatic Organisms
volume
Volume 24
issue
Issue 2
start page
99
end page
105
date of issued
1996-02-01
publisher
Inter-Research
issn
0177-5103
1616-1580
ncid
publisher doi
language
eng
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
format
application/pdf
text version
publisher
rights
Copyright (c) 1996 Inter-Research.
relation url
department
Graduate School of Biosphere Science