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ID 25584
file
creator
Fukui, Yukio
subject
inverse PCR
corticotropin-releasing hormone precursor
teleost
Halichoeres tenuispinnis
NDC
Fishing industry. Fisheries
abstract
We designed a new inverse PCR protocol combined with switching mechanism at 5′ end of RNA transcript (SMART) technology, and applied it to the cloning of teleost corticotropin-releasing hormone precursor cDNA. Due to the advantages of both techniques, this method can efficiently amplify the complete 5′- and 3′-ends of cDNA in a single reaction, and might prove to be an alternative to the conventional rapid amplification of cDNA ends (RACE) approaches.
journal title
Bioscience, Biotechnology, and Biochemistry
volume
Volume 70
issue
Issue 8
start page
1983
end page
1986
date of issued
2006
publisher
Japan Society for Bioscience, Biotechnology, and Agrochemistry
issn
0916-8451
ncid
publisher doi
language
eng
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
format
application/pdf
text version
publisher
rights
Copyright (c) 2006 Japan Society for Bioscience, Biotechnology, and Agrochemistry
relation url
department
Graduate School of Biosphere Science