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ID 34921
file
creator
Fujito, Naoya
Samura, Osamu
Miharu, Norio
Tanigawa, Miho
subject
Quantitative analysis of placental mRNA
Noninvasive prediction markers for preeclampsia
Placenta-specific 1 (PLAC1)
Glial cells-missing 1 (GCM1)
NDC
Medical sciences
abstract
In this study we have investigated whether quantitative analysis of placental mRNAs in maternal plasma provides a way to monitor placental status. We measured plasma concentrations of human chorionic gonadotropin /)-subunit (/3hCG) and human placental lactogen (hPL) mRNAs as previously reported mRNAs and pregnancy associated plasma protein A (PAPP-A), placenta-specific 1 (PLACl) and glial cells-missing 1 (GCMl) mRNAs, which have not been measured during the course of normal pregnancy. Firstly, peripheral blood was obtained at various times from healthy pregnant women to clarify the time course of placental mRNAs. Secondly, blood was obtained from women with pre-eclampsia and gestational age-matched controls to examine whether placental mRNAs change in pre-eclampsia. Plasma was separated from these samples for extraction of RNA, followed by reverse transcription polymerse chain reaction analysis. Median concentrations of PLACl and GCMl mRNA in plasma of pre-eclamptic subjects respectively were 1625 and 2141 copies/ml, significantly higher than 195 and 881 copies/ml, the values for controls (Mann-Whitney test, p<0.001). No significant difference was seen in hPL, f3hCG, or PAPP-A mRNA concentration between pre-eclamptic and control groups. Plasma PLACl and GCMl mRNAs appear promising as noninvasively measurable molecular markers for pre-eclampsia.
journal title
Hiroshima Journal of Medical Sciences
volume
Volume 55
issue
Issue 1
start page
9
end page
15
date of issued
2006-03
publisher
Hiroshima University Medical Press
issn
0018-2052
ncid
language
eng
nii type
Departmental Bulletin Paper
HU type
Departmental Bulletin Papers
DCMI type
text
format
application/pdf
text version
publisher
rights
(c) Hiroshima University Medical Press.
department
Graduate School of Biomedical Science
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