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ID 28861
file
creator
Yanase, Yuhki
Uchida, Kazue
Ishii, Kaori
Oomizu, Souichi
Suzuki, Hidenori
Mihara, Shoji
Onishi, Nobukazu
Kameyoshi, Yoshikazu
subject
Fucoidan
B cells
T cells
IgE
Ovalbumin (OVA)
NDC
Medical sciences
abstract
We previously reported that fucoidan, a dietary fiber purified from seaweed, inhibited IgE production by B cells in vitro. In this study, we examined the effect of fucoidan on IgE production in vivo. The OVA-induced increase of plasma IgE was significantly suppressed when fucoidan was intraperitoneally, but not orally, administered prior to the first immunization with OVA. The production of IL-4 and IFN-gamma in response to OVA in spleen cells isolated from OVA-sensitized mice treated with fucoidan in vivo was lower than that from mice treated without fucoidan. Moreover, the flow cytometric analysis and ELISpot assay revealed that the administration of fucoidan Suppressed a number of IgE-expressing and IgE-secreting B cells, respectively. These results indicate that fucoidan inhibits the increase of plasma IgE through the suppression of IgE-producing B cell population, and the effect of fucoidan in VIVO is Crucially dependent on the route and timing of its administration.
journal title
Biochemical and Biophysical Research Communications
volume
Volume 387
issue
Issue 3
start page
435
end page
439
date of issued
2009-09-25
publisher
Academic Press Inc
Elsevier Science
issn
0006-291X
ncid
publisher doi
language
eng
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
format
application/pdf
text version
author
rights
Copyright (c) 2009 Elsevier Inc.
relation url
department
Graduate School of Biomedical Science