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ID 17059
file
creator
Yamashita, Yusuke
Tategaki, Airo
Ogawa, Mari
Nishida, Kensho
Akita, Sachiko
Matsuda, Haruo
subject
chicken
chicken blastodermal cells
cytokine
ES cells
LIF
mAb
MEK1/2
STAT3
NDC
Biology
abstract
Leukemia-inhibitory factor (LIF) is indispensable for maintaining the undifferentiated state when propagating mouse embryonic stem (ES) cells. We previously cloned chicken LIF (chLIF) cDNA and demonstrated that it maintained chicken ES cell cultures in an undifferentiated state. Here, we developed two monoclonal antibodies, HUL-1 and HUL-2, against chLIF, which specifically recognized recombinant chLIF (rchLIF) produced by Escherichia coli and Chinese hamster ovary K1 cells, in enzyme-linked immunosorbent assays and Western blot analysis. In addition, HUL-2 inhibited the phosphorylation of signal transducer and activator of transcription 3 by rchLIF in chicken blastodermal cells (CBCs), but not that of mitogen-activated protein kinase kinase. Furthermore, the addition of HUL-2 to CBC cultures resulted in embryoid bodies forming earlier than in normal cultures. These results indicated that HUL-2 recognized not only rchLIF but also native chLIF, and suggested that CBCs in culture produce LIF, which functions in autocrine signaling.
journal title
Developmental and Comparative Immunology
volume
Volume 30
issue
Issue 5
start page
513
end page
522
date of issued
2006
publisher
Elsevier
issn
0145-305X
ncid
publisher doi
pubmed id
language
eng
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
format
application/pdf
text version
author
rights
Copyright (c) 2006 Elsevier Ltd.
relation is version of URL
http://dx.doi.org/10.1016/j.dci.2005.07.003
department
Graduate School of Biosphere Science