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ID 34799
file
creator
Elteliba, Hani A
subject
Acerola (Malpighia glabra)
Ascorbic acid
Monodehydroascorbate reductase
Salt stress
Transgenic
NDC
Biology
abstract
Monodehydroascorbate reductase (MDHAR, EC 1.6.5.4) is a key enzyme of the ascorbate (AsA)-glutathione cycle that maintains reduced pools of AsA and serves as an important antioxidative enzyme. Previously, we have cloned MDHAR cDNA from acerola (Malpighia glabra), a plant that accumulates abundant amount of AsA. In this study, MDHAR cDNA from acerola was introduced into tobacco plants using an Agrobacterium-mediated gene delivery system. Transgenic tobacco plants accumulated greater amounts of AsA and showed higher MDHAR activity than the control plants. Lipid peroxidation and chlorophyll degradation, which were stimulated in control plants, were restrained in transgenic plants subjected to salt stress. These results indicate that overexpression of acerola MDHAR imparts greater tolerance to salt stress. (C) 2011 SAAB. Published by Elsevier B.V. All rights reserved.
journal title
South African Journal of Botany
volume
Volume 78
start page
295
end page
301
date of issued
2012
publisher
Elsevier Science BV
issn
0254-6299
ncid
publisher doi
language
eng
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
format
application/pdf
text version
author
rights
(c) 2012 Elsevier B.V. All rights reserved.
relation url
department
Graduate School of Biosphere Science