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ID 38130
file
creator
Ochiai, Atsushi
Kameda, Takashi
Takanashi, Atsushi
Takekura, Naoki
Tahara, Eiichi
subject
Phorbol ester
Protein kinase C
Gastric cancer cell
EGF receptor
NDC
Medical sciences
abstract
Tumor promoting phorbol esters, 12-O-tetradecanoylphorbol-13-acetate (TPA) and phorbol-12, 13-dibutyrate (PDBu), significantly enhanced the growth of human gastric cancer cell line TMK-1, whilst activating protein kinase C. The time course of 125I-epidermal growth factor (EGF) binding to TMK-1 cells after TPA treatment showed a decrease in the number of EGF receptors on TMK-1 cells within 3 hr. Autophosphorylation of EGF receptor decreased in accordance with the decrease of EGF binding by TPA treatment. Scatchard plot analysis of TMK-1 cells after TPA treatment showed that high affinity EGF receptor disappeared at 3hr but the number of EGF receptors increased at 24 hr. These findings suggest that tumor promoting phorbol esters stimulate the cell growth through activation of protein kinase C and modification of EGF receptor of human gastric cancer cell line TMK-1.
description
This research was supported in part by Grants-in Aid for Cancer Research from the Ministry of Education, Science and Culture of Japan and from the Ministry of Health and Welfare for a Comprehensive 10-Year Strategy for Cancer Control, Japan.
journal title
Hiroshima Journal of Medical Sciences
volume
Volume 38
issue
Issue 4
start page
191
end page
195
date of issued
1989-12
publisher
Hiroshima University Medical Press
issn
0018-2052
ncid
pubmed id
language
eng
nii type
Departmental Bulletin Paper
HU type
Departmental Bulletin Papers
DCMI type
text
format
application/pdf
text version
publisher
department
Graduate School of Biomedical Science
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